552 Skin metaproteomics a key functional approach to study skin healthiness and resilience

Our skin health and its resilience against assaults (pollution, climatic conditions, pathogens) rests mainly on the epidermis microbiome. Indeed, is colonized by commensal beneficial microorganisms called This complex ecosystem a shield for our ensuring protective functions while educating immune system. Unfortunately, when both microbiome are fragilized diseases occur. Thus, taking care of crucial to preserve healthiness. Therefore, studying with adapted methodologies clinical sampling might help gain valuable insight during development solutions dedicated health. The option we choose metaproteomics, which has only been used in gut microbiota field. In contrast metagenomics, metaproteomics provide descriptive functional insights simultaneously these two allies interplay: aim this work demonstrate randomized study, efficiency natural active ingredient (obtained from white biotechnology) healthiness using metaproteomics. Skin swabs were sampled women’s faces before after use cosmetic formula. results that wasn’t harmful microbiome, keeping composition stable. We identified 20042 peptides belonging 4785 proteins. Among them, 343 proteins significantly modulated (from human, fungi bacterial origins) impacting positively anti-oxidant, proteostasis, metabolism, anti-inflammation extra-cellular matrix pathways. Finally, highlight powerful technology allowing modulating expression at keratinocytes levels lead clearly visible outcomes: improvement wrinkles, mechanical properties barrier function.